t100tm thermal cycler pcr machine 6 (Bio-Rad)
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t100tm thermal cycler pcr machine 6
T100tm Thermal Cycler Pcr Machine 6, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 13837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t100tm+thermal+cycler+pcr+machine+6/T100+Thermal+Cycler/us12590273-1278-169-168
Average 99 stars, based on 13837 article reviews
T100tm Thermal Cycler Pcr Machine 6, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 13837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t100tm+thermal+cycler+pcr+machine+6/T100+Thermal+Cycler/us12590273-1278-169-168
Average 99 stars, based on 13837 article reviews
t100tm thermal cycler pcr machine 6 - by Bioz Stars,
2026-10
99/100 stars
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CMC:Article Title: Stabilized glycoside hydrolase variants Article Snippet: C. CMC Activity Assay Chemicals: CMC: Sodium carboxymethyl cellulose (Sigma C5678) K-Na-Tartrate: Merck 8087 β-glucosidase Megazyme (Thermotoga maritima; accession number Q08638, Megazyme Cat. No. E-BGOSTM) diluted to 0.1 mg/ml (specific activity 70 U/mg and activity in product ̃460 U/mL→6.57 mg/mL) PAHBAH 4-Hydroxybenzhydrazide (Sigma H9882) NaOH sodium hydroxide (J.T. .. Baker 0402.1000) HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Sigma H3375 Reagents: Assay Buffer: 50 mM HEPES, pH 8 CMC Substrate: 1.25 g CMC/100 mL Assay Buffer, mixed 1 hr before use PAHBAH Buffer: 50 g/L K-Na-tartrate+20 g/L NaOH PAHBAH Reagent: 15 mg/mL PAHBAH in PAHBAH Buffer B-glucosidase Solution: 0.1 mg/ml B-glucosidase in Assay Buffer Procedure: 1) 160 μL CMC Substrate is pipetted into new 96-well microplates (Thermo ScientificTM cat. No. 269620) 2)20 μL diluted supernatant from step B is added together with 20 UL B-glucosidase Solution 3) The plate was sealed with heat seal (Thermo ScientificTM Adhesive PCR Plate Seals AB0558), and incubated for 45 minutes at 40° C. 4) After reaction, 100 μL from each well is transferred to ThermoFast 96 Non-Skirted® PCR plate (Thermo ScientificTM cat. No. AB-0600) followed by 75 μL PAHBAH Reagent 5) The plate from (4) is then sealed with sealing foil (Greiner bio-one platesealer, Cat. No. 676001) and incubated at 95° C. for 10 minutes followed by cooling at 10° C. for 5 minutes in BioRad Article Title: Stabilized glycoside hydrolase variants Article Snippet: B. CMC Activity Assay Chemicals: CMC: Sodium carboxymethyl cellulose (Sigma C5678) K—Na-Tartrate: Merck 8087 β-glucosidase Megazyme (Thermotoga maritima; accession number Q08638, Megazyme Cat. No. E-BGOSTM) diluted to 0.1 mg/ml (specific activity 70 U/mg and activity in product ̃460 U/mL→6.57 mg/mL) PAHBAH 4-Hydroxybenzhydrazide (Sigma H9882) NaOH sodium hydroxide (J.T. .. Baker 0402.1000) HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Sigma H3375 Reagents: Assay Buffer: 50 mM HEPES, pH 8 CMC Substrate: 1.25 g CMC/100 mL Assay Buffer, mixed 1 hr before use PAHBAH Buffer: 50 g/L K-Na-tartrate+20 g/L NaOH PAHBAH Reagent: 15 mg/mL PAHBAH in PAHBAH Buffer β-glucosidase Solution: 0.1 mg/ml β-glucosidase in Assay Buffer Procedure: 1) 160 μL CMC Substrate is pipetted into new 96-well microplates (Thermo Scientific cat. No. 269620) 2)20 μL diluted supernatant from step A is added together with 20 μL B-glucosidase Solution 3) The plate was sealed with heat seal (Thermo Scientific Adhesive PCR Plate Seals AB0558), and incubated for 45 minutes at 40° C. 4) After reaction, 100 μL from each well is transferred to ThermoFast 96 Non-Skirted® PCR plate (Thermo Scientific cat. No. AB-0600) followed by 75 μL PAHBAH Reagent 5) The plate from (4) is then sealed with sealing foil (Greiner bio-one platesealer, Cat. No. 676001) and incubated at 95° C. for 10 minutes followed by cooling at 10° C. for 5 minutes in BioRad Adhesive:Article Title: Stabilized glycoside hydrolase variants Article Snippet: C. CMC Activity Assay Chemicals: CMC: Sodium carboxymethyl cellulose (Sigma C5678) K-Na-Tartrate: Merck 8087 β-glucosidase Megazyme (Thermotoga maritima; accession number Q08638, Megazyme Cat. No. E-BGOSTM) diluted to 0.1 mg/ml (specific activity 70 U/mg and activity in product ̃460 U/mL→6.57 mg/mL) PAHBAH 4-Hydroxybenzhydrazide (Sigma H9882) NaOH sodium hydroxide (J.T. .. Baker 0402.1000) HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Sigma H3375 Reagents: Assay Buffer: 50 mM HEPES, pH 8 CMC Substrate: 1.25 g CMC/100 mL Assay Buffer, mixed 1 hr before use PAHBAH Buffer: 50 g/L K-Na-tartrate+20 g/L NaOH PAHBAH Reagent: 15 mg/mL PAHBAH in PAHBAH Buffer B-glucosidase Solution: 0.1 mg/ml B-glucosidase in Assay Buffer Procedure: 1) 160 μL CMC Substrate is pipetted into new 96-well microplates (Thermo ScientificTM cat. No. 269620) 2)20 μL diluted supernatant from step B is added together with 20 UL B-glucosidase Solution 3) The plate was sealed with heat seal (Thermo ScientificTM Adhesive PCR Plate Seals AB0558), and incubated for 45 minutes at 40° C. 4) After reaction, 100 μL from each well is transferred to ThermoFast 96 Non-Skirted® PCR plate (Thermo ScientificTM cat. No. AB-0600) followed by 75 μL PAHBAH Reagent 5) The plate from (4) is then sealed with sealing foil (Greiner bio-one platesealer, Cat. No. 676001) and incubated at 95° C. for 10 minutes followed by cooling at 10° C. for 5 minutes in BioRad Article Title: Stabilized glycoside hydrolase variants Article Snippet: B. CMC Activity Assay Chemicals: CMC: Sodium carboxymethyl cellulose (Sigma C5678) K—Na-Tartrate: Merck 8087 β-glucosidase Megazyme (Thermotoga maritima; accession number Q08638, Megazyme Cat. No. E-BGOSTM) diluted to 0.1 mg/ml (specific activity 70 U/mg and activity in product ̃460 U/mL→6.57 mg/mL) PAHBAH 4-Hydroxybenzhydrazide (Sigma H9882) NaOH sodium hydroxide (J.T. .. Baker 0402.1000) HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Sigma H3375 Reagents: Assay Buffer: 50 mM HEPES, pH 8 CMC Substrate: 1.25 g CMC/100 mL Assay Buffer, mixed 1 hr before use PAHBAH Buffer: 50 g/L K-Na-tartrate+20 g/L NaOH PAHBAH Reagent: 15 mg/mL PAHBAH in PAHBAH Buffer β-glucosidase Solution: 0.1 mg/ml β-glucosidase in Assay Buffer Procedure: 1) 160 μL CMC Substrate is pipetted into new 96-well microplates (Thermo Scientific cat. No. 269620) 2)20 μL diluted supernatant from step A is added together with 20 μL B-glucosidase Solution 3) The plate was sealed with heat seal (Thermo Scientific Adhesive PCR Plate Seals AB0558), and incubated for 45 minutes at 40° C. 4) After reaction, 100 μL from each well is transferred to ThermoFast 96 Non-Skirted® PCR plate (Thermo Scientific cat. No. AB-0600) followed by 75 μL PAHBAH Reagent 5) The plate from (4) is then sealed with sealing foil (Greiner bio-one platesealer, Cat. No. 676001) and incubated at 95° C. for 10 minutes followed by cooling at 10° C. for 5 minutes in BioRad Polymerase Chain Reaction:Article Title: Stabilized glycoside hydrolase variants Article Snippet: C. CMC Activity Assay Chemicals: CMC: Sodium carboxymethyl cellulose (Sigma C5678) K-Na-Tartrate: Merck 8087 β-glucosidase Megazyme (Thermotoga maritima; accession number Q08638, Megazyme Cat. No. E-BGOSTM) diluted to 0.1 mg/ml (specific activity 70 U/mg and activity in product ̃460 U/mL→6.57 mg/mL) PAHBAH 4-Hydroxybenzhydrazide (Sigma H9882) NaOH sodium hydroxide (J.T. .. Baker 0402.1000) HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Sigma H3375 Reagents: Assay Buffer: 50 mM HEPES, pH 8 CMC Substrate: 1.25 g CMC/100 mL Assay Buffer, mixed 1 hr before use PAHBAH Buffer: 50 g/L K-Na-tartrate+20 g/L NaOH PAHBAH Reagent: 15 mg/mL PAHBAH in PAHBAH Buffer B-glucosidase Solution: 0.1 mg/ml B-glucosidase in Assay Buffer Procedure: 1) 160 μL CMC Substrate is pipetted into new 96-well microplates (Thermo ScientificTM cat. No. 269620) 2)20 μL diluted supernatant from step B is added together with 20 UL B-glucosidase Solution 3) The plate was sealed with heat seal (Thermo ScientificTM Adhesive PCR Plate Seals AB0558), and incubated for 45 minutes at 40° C. 4) After reaction, 100 μL from each well is transferred to ThermoFast 96 Non-Skirted® PCR plate (Thermo ScientificTM cat. No. AB-0600) followed by 75 μL PAHBAH Reagent 5) The plate from (4) is then sealed with sealing foil (Greiner bio-one platesealer, Cat. No. 676001) and incubated at 95° C. for 10 minutes followed by cooling at 10° C. for 5 minutes in BioRad Article Title: Stabilized glycoside hydrolase variants Article Snippet: B. CMC Activity Assay Chemicals: CMC: Sodium carboxymethyl cellulose (Sigma C5678) K—Na-Tartrate: Merck 8087 β-glucosidase Megazyme (Thermotoga maritima; accession number Q08638, Megazyme Cat. No. E-BGOSTM) diluted to 0.1 mg/ml (specific activity 70 U/mg and activity in product ̃460 U/mL→6.57 mg/mL) PAHBAH 4-Hydroxybenzhydrazide (Sigma H9882) NaOH sodium hydroxide (J.T. .. Baker 0402.1000) HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Sigma H3375 Reagents: Assay Buffer: 50 mM HEPES, pH 8 CMC Substrate: 1.25 g CMC/100 mL Assay Buffer, mixed 1 hr before use PAHBAH Buffer: 50 g/L K-Na-tartrate+20 g/L NaOH PAHBAH Reagent: 15 mg/mL PAHBAH in PAHBAH Buffer β-glucosidase Solution: 0.1 mg/ml β-glucosidase in Assay Buffer Procedure: 1) 160 μL CMC Substrate is pipetted into new 96-well microplates (Thermo Scientific cat. No. 269620) 2)20 μL diluted supernatant from step A is added together with 20 μL B-glucosidase Solution 3) The plate was sealed with heat seal (Thermo Scientific Adhesive PCR Plate Seals AB0558), and incubated for 45 minutes at 40° C. 4) After reaction, 100 μL from each well is transferred to ThermoFast 96 Non-Skirted® PCR plate (Thermo Scientific cat. No. AB-0600) followed by 75 μL PAHBAH Reagent 5) The plate from (4) is then sealed with sealing foil (Greiner bio-one platesealer, Cat. No. 676001) and incubated at 95° C. for 10 minutes followed by cooling at 10° C. for 5 minutes in BioRad Incubation:Article Title: Stabilized glycoside hydrolase variants Article Snippet: C. CMC Activity Assay Chemicals: CMC: Sodium carboxymethyl cellulose (Sigma C5678) K-Na-Tartrate: Merck 8087 β-glucosidase Megazyme (Thermotoga maritima; accession number Q08638, Megazyme Cat. No. E-BGOSTM) diluted to 0.1 mg/ml (specific activity 70 U/mg and activity in product ̃460 U/mL→6.57 mg/mL) PAHBAH 4-Hydroxybenzhydrazide (Sigma H9882) NaOH sodium hydroxide (J.T. .. Baker 0402.1000) HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Sigma H3375 Reagents: Assay Buffer: 50 mM HEPES, pH 8 CMC Substrate: 1.25 g CMC/100 mL Assay Buffer, mixed 1 hr before use PAHBAH Buffer: 50 g/L K-Na-tartrate+20 g/L NaOH PAHBAH Reagent: 15 mg/mL PAHBAH in PAHBAH Buffer B-glucosidase Solution: 0.1 mg/ml B-glucosidase in Assay Buffer Procedure: 1) 160 μL CMC Substrate is pipetted into new 96-well microplates (Thermo ScientificTM cat. No. 269620) 2)20 μL diluted supernatant from step B is added together with 20 UL B-glucosidase Solution 3) The plate was sealed with heat seal (Thermo ScientificTM Adhesive PCR Plate Seals AB0558), and incubated for 45 minutes at 40° C. 4) After reaction, 100 μL from each well is transferred to ThermoFast 96 Non-Skirted® PCR plate (Thermo ScientificTM cat. No. AB-0600) followed by 75 μL PAHBAH Reagent 5) The plate from (4) is then sealed with sealing foil (Greiner bio-one platesealer, Cat. No. 676001) and incubated at 95° C. for 10 minutes followed by cooling at 10° C. for 5 minutes in BioRad Article Title: Stabilized glycoside hydrolase variants Article Snippet: B. CMC Activity Assay Chemicals: CMC: Sodium carboxymethyl cellulose (Sigma C5678) K—Na-Tartrate: Merck 8087 β-glucosidase Megazyme (Thermotoga maritima; accession number Q08638, Megazyme Cat. No. E-BGOSTM) diluted to 0.1 mg/ml (specific activity 70 U/mg and activity in product ̃460 U/mL→6.57 mg/mL) PAHBAH 4-Hydroxybenzhydrazide (Sigma H9882) NaOH sodium hydroxide (J.T. .. Baker 0402.1000) HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Sigma H3375 Reagents: Assay Buffer: 50 mM HEPES, pH 8 CMC Substrate: 1.25 g CMC/100 mL Assay Buffer, mixed 1 hr before use PAHBAH Buffer: 50 g/L K-Na-tartrate+20 g/L NaOH PAHBAH Reagent: 15 mg/mL PAHBAH in PAHBAH Buffer β-glucosidase Solution: 0.1 mg/ml β-glucosidase in Assay Buffer Procedure: 1) 160 μL CMC Substrate is pipetted into new 96-well microplates (Thermo Scientific cat. No. 269620) 2)20 μL diluted supernatant from step A is added together with 20 μL B-glucosidase Solution 3) The plate was sealed with heat seal (Thermo Scientific Adhesive PCR Plate Seals AB0558), and incubated for 45 minutes at 40° C. 4) After reaction, 100 μL from each well is transferred to ThermoFast 96 Non-Skirted® PCR plate (Thermo Scientific cat. No. AB-0600) followed by 75 μL PAHBAH Reagent 5) The plate from (4) is then sealed with sealing foil (Greiner bio-one platesealer, Cat. No. 676001) and incubated at 95° C. for 10 minutes followed by cooling at 10° C. for 5 minutes in BioRad |